Liquid Nitrogen Preservation of Standard Inoculum: Gas-Phase Storage
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چکیده
منابع مشابه
Capacitive gas-phase detection in liquid nitrogen
The main and upper stages of heavy lift launchers for space applications are often fuelled by cryogenic liquids. In order to enable the re-ignition of a cryogenic upper stage for orbital changes, it is crucial to study the behaviour of these fluids in microgravity. As gaseous bubbles entering the fuel lines of the engine can cause the destruction of the engine, these bubbles are a risk for the ...
متن کاملPreservation of T2 Bacteriophage with Liquid Nitrogen.
There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially pur...
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In recent years some attention has been focused on the low temperature storage of various protozoa. The purpose of this work was to confirm that Trichomonas vaginalis can be preserved for several months at a temperature of -196°C. in liquid nitrogen. Weinman and McAllister (1947), who successfully preserved a number of pathogenic protozoa, failed to preserve T. vaginalis, and only a proportion ...
متن کاملThe preservation of gonococci in liquid nitrogen.
Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described...
متن کاملLiquid Nitrogen Storage of Haemoglobin Variants.
Received for publication 26 April 1963. is thawed immediately after freezing a precipitate of denatured protein can be seen in the solution. We have recently been investigating the storage of human red cells in liquid nitrogen for serological purposes (Huntsman, Hum, Ikin, Lehmann, and Liddell, 1962). We thought that this technique might be useful for the preservation of haemoglobin as well as ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1968
ISSN: 0003-6919
DOI: 10.1128/am.16.6.912-916.1968